ICA regulates expression of genes in the type I interferon (IFN1) and circadian rhythm pathways. (A) Venn diagram indicating the schema for identification of both GVHD-dependent and GVHD- and ICA-dependent gene expression changes assessed 21 days after B6 → B10.BR transplant. (B) Hierarchical clustering of 97 z-score normalized GVHD- and ICA-dependent genes in all 4 experimental conditions (TCD-BM or TCD-BM + T treated with either VEH or ICA). Each column represents averaged z-score data from n = 4 (TCD-BM) or n = 5 (TCD-BM + T) animals. Group A represents genes downregulated by ICA in TCD-BM + T transplanted animals. Group B represents genes upregulated by ICA in TCD-BM + T transplanted animals. (C) Principal component analysis of GVHD- and ICA-dependent genes; n = 4 (TCD-BM) or n = 5 (TCD-BM + T). (D) Gene ontology analysis (GoAmigo) of group A and group B GVHD- and ICA-dependent genes. Statistics: 1-way and 2-way ANOVA tests were performed to determine significant differential expression of genes among groups and identify common genes. Differential expression filtering was performed using an unadjusted P value <.05 and absolute fold change >1.5.