Bcl11a and human γ-globin transgene levels remain unchanged in HRI knockout Townes mice. (A) Complete blood cell count of HRI^+/+, HRI^+/−, and HRI^−/− Townes β^Aβ^S mice. Results are shown as mean ± standard deviation (SD; n = 3). (B) HRI (left), Bcl11a (middle), and γ-globin (right) mRNA levels as measured by quantitative reverse transcription polymerase chain reaction (RT-qPCR) of sorted CD71⁺Ter119⁺ bone marrow cells in HRI^+/+, HRI^+/−, and HRI^−/− Townes β^Aβ^S mice. HRI and Bcl11a are displayed as absolute values normalized to RPS18, whereas γ-globin is displayed as percentage of total β-like globin transcripts. Results are shown as mean ± SD (n = 3). *P < .05, **P < .01 by Student t test. MCV, mean corpuscular volume; MCH, mean corpuscular hemoglobin; n.s., not significant; PLT, platelet count; WBC, white blood cell count.