Simultaneous lack of Jagged1 in the BM stroma and Notch1 in HSCs of mixed BM chimeras does not affect their self-renewal potential. (A) Mixed BM chimeric mice analyzed 14 to 15 weeks after reconstitution with a 1:2 mixture of B6-SJL (CD45.1⁺) and either J1^lox/lox or J1^-/- (CD45.2⁺) BM-derived populations. Percentage of CD45.2⁺ BM cells, CD45.2⁺Lin^- cells, and HSCs (CD117⁺Sca1⁺) gated on lin^-CD45.2⁺ cells from either J1^lox/lox (▪) or J1^-/- mice (▦). The bars represent mean ± SD (n = 8). (B) Mixed BM chimeric mice using either J1^lox/lox or induced J1^-/- recipients (both CD45.2⁺) analyzed 8 to 20 weeks after reconstitution with a 1:1 mixture of C57BL/6 (CD45.2⁺) and either N1^lox/lox or N1^-/- (CD45.1⁺) BM-derived populations. (Top row) Percentage of CD45.1⁺ BM cells, CD45.1⁺Lin^- cells, and HSCs (CD117⁺Sca1⁺), long-term-HSCs (CD117⁺Sca1⁺CD135^-), short-term-HSCs (CD117⁺Sca1⁺CD135⁺), CMPs (CD117⁺Sca1^-), and CLPs (CD117^intSca1^int) gated on lin^-CD45.1⁺ BM cells. (Bottom row) B cells (B220⁺), immature myeloid cells (CD11b⁺/Gr1^lo/int), eosinophils (CD11b⁺/Gr1⁺), early erythroblasts (Ter119⁺) gated on CD45.1⁺ cells, and percentage of CD45.1⁺ cells in the thymus. Most of the thymic CD45.1⁺ cells derived from N1^-/- BM cells are B cells. Control into control (▪; n = 8), control into J1^-/- (▨; n = 3), N1^-/- into control (▥; n = 7), or N1^-/- into J1^-/- (□; n = 14) mixed BM chimeras. The bars represent mean ± SD. (C) PCR analysis of the deletion of the N1 gene using genomic DNA from CD45.1⁺ HSCs sorted 8 weeks after transplantation of the indicated chimeric mice.