Figure 2. Ang1 and Ang2 induce neutrophil adhesion to HUVECs. Neutrophil adhesion to HUVECs was determined by cell surface adhesion assay under static conditions. PBS was used as a control. Due to slight variations in basal neutrophil adhesion between different experiments, we reported our data as relative neutrophil adhesion (%). Neutrophil recruitment induced by Ang1 (A) and Ang2 (B), both at 7.5 minutes, was determined at different concentrations (10–12 to 10–8 M; left panels) and in function of time at 10–9 M (5 to 15 minutes; right panels). Data are means ± SEM of at least 12 experiments; *P = .01 compared with PBS.
Figure 2.

Ang1 and Ang2 induce neutrophil adhesion to HUVECs. Neutrophil adhesion to HUVECs was determined by cell surface adhesion assay under static conditions. PBS was used as a control. Due to slight variations in basal neutrophil adhesion between different experiments, we reported our data as relative neutrophil adhesion (%). Neutrophil recruitment induced by Ang1 (A) and Ang2 (B), both at 7.5 minutes, was determined at different concentrations (10–12 to 10–8 M; left panels) and in function of time at 10–9 M (5 to 15 minutes; right panels). Data are means ± SEM of at least 12 experiments; *P = .01 compared with PBS.

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