Figure 6. Restimulation of suppressor lines reveals cytokine production defects and cell surface LAP expression. Levels of cytokine produced in supernatants 48 hours after restimulation. (A) Cell lines were reactivated with anti-CD3/CD28 beads. (B) Cells were reactivated with PMA/ionomycin. Control CD25--derived cell lines are indicated by blue bars and CD25+-derived cell lines by burgundy bars. Cell surface activation antigen expression 24 hours after restimulation with anti-CD3/CD28 beads. (C) Expression of CD69, OX40 (CD134), and GITR is intact. (D) Cell surface LAP expression is specific for CD25+-derived cell lines. Resting cell lines are indicated by blue lines and restimulated cell lines by red lines. Resting cell lines were negative for these activation antigens prior to restimulation.
Figure 6.

Restimulation of suppressor lines reveals cytokine production defects and cell surface LAP expression. Levels of cytokine produced in supernatants 48 hours after restimulation. (A) Cell lines were reactivated with anti-CD3/CD28 beads. (B) Cells were reactivated with PMA/ionomycin. Control CD25--derived cell lines are indicated by blue bars and CD25+-derived cell lines by burgundy bars. Cell surface activation antigen expression 24 hours after restimulation with anti-CD3/CD28 beads. (C) Expression of CD69, OX40 (CD134), and GITR is intact. (D) Cell surface LAP expression is specific for CD25+-derived cell lines. Resting cell lines are indicated by blue lines and restimulated cell lines by red lines. Resting cell lines were negative for these activation antigens prior to restimulation.

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