Figure 5. RA regulation of CHOP does not require new protein synthesis. The effect of a protein synthesis inhibitor, cycloheximide (CHX), on retinoid-dependent CHOP mRNA expression. NB4 cells were incubated with CHX (10 μg/mL) alone, CHX and ATRA (5 × 10-7 M), or ATRA alone for 0, 1, and 4 hours. Northern blot analysis was carried out using total RNA (30 μg/lane) and hybridized with 32P-labeled full-length CHOP cDNA probe. The same blot was rehybridized for GAPDH to ensure equal RNA loading.
Figure 5.

RA regulation of CHOP does not require new protein synthesis. The effect of a protein synthesis inhibitor, cycloheximide (CHX), on retinoid-dependent CHOP mRNA expression. NB4 cells were incubated with CHX (10 μg/mL) alone, CHX and ATRA (5 × 10-7 M), or ATRA alone for 0, 1, and 4 hours. Northern blot analysis was carried out using total RNA (30 μg/lane) and hybridized with 32P-labeled full-length CHOP cDNA probe. The same blot was rehybridized for GAPDH to ensure equal RNA loading.

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