Polypeptide plots from patient no. 291 (no complications) and patient no. 293 (GVHD). Polypeptide plots (polypeptide mass over CE migration time) are shown for 2 patients, no. 291 (A) and no. 294 (B), at an early posttransplantation period. While patient 291 had no complications in the observation period, patient 294 was diagnosed with GVHD on day +14. To visualize the search for biomarkers and to show the differences between these patients, panel C shows a magnified view of the plots obtained at different days after HSCT (0 to +13/+35 as shown by the black arrow) of the polypeptide 1.85 kDa/34 minutes. This polypeptide appears in the samples of patient 293, as early as day +1, showing increasing intensity over the progression of GVHD (arrow, starting at day +15, indicating the clinical diagnosis of GVHD).