Figure 6. The FOXO3a target BimS is down-regulated in cells inducibly or constitutively expressing NPM-ALK. (A) Western blot analysis of Bim-1 expression in TonBaF.1 cells inducibly expressing NPM-ALK. Uninduced samples were harvested from cells starved of IL-3 for 17 hours. +Dox 24hr samples were harvested from cells starved of IL-3 for 17 hours and induced with 2 μg/mL doxycycline for 24 hours. Whole-cell lysates were fractionated by SDS-PAGE and immunoblotted with Bim-1-specific antibody. The membrane was stripped and reblotted with Rack1-specific antibody to confirm equal loading. (B) Western blot analysis of BimS expression levels in Ba/F3 cells stably transformed with NPM-ALK. Whole-cell lysates of MSCV- and MSCV-NPM-ALK-transduced Ba/F3 cells were harvested after a 17-hour IL-3 starvation, fractionated by SDS-PAGE, and immunoblotted with Bim-1-specific antibody. The membrane was stripped and reblotted with Rack1-specific antibody to confirm equal loading.
Figure 6.

The FOXO3a target BimS is down-regulated in cells inducibly or constitutively expressing NPM-ALK. (A) Western blot analysis of Bim-1 expression in TonBaF.1 cells inducibly expressing NPM-ALK. Uninduced samples were harvested from cells starved of IL-3 for 17 hours. +Dox 24hr samples were harvested from cells starved of IL-3 for 17 hours and induced with 2 μg/mL doxycycline for 24 hours. Whole-cell lysates were fractionated by SDS-PAGE and immunoblotted with Bim-1-specific antibody. The membrane was stripped and reblotted with Rack1-specific antibody to confirm equal loading. (B) Western blot analysis of BimS expression levels in Ba/F3 cells stably transformed with NPM-ALK. Whole-cell lysates of MSCV- and MSCV-NPM-ALK-transduced Ba/F3 cells were harvested after a 17-hour IL-3 starvation, fractionated by SDS-PAGE, and immunoblotted with Bim-1-specific antibody. The membrane was stripped and reblotted with Rack1-specific antibody to confirm equal loading.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal