Figure 3. Coimmunoprecipitation of hsp90 and hsp70 with KIT protein. KIT protein was immunoprecipitated from HMC-1.1 and HMC-1.2 cells and associated hsp90 and hsp70 proteins were probed by Western blot analysis (A). Cells were treated with 1 μM 17-AAG for 1 hour. Protein G-Sepharose beads alone were used to exclude the possibility of nonspecific binding. Treatment with 17-AAG for 1 hour was chosen because of minimal change in total KIT levels during that time (B).
Figure 3.

Coimmunoprecipitation of hsp90 and hsp70 with KIT protein. KIT protein was immunoprecipitated from HMC-1.1 and HMC-1.2 cells and associated hsp90 and hsp70 proteins were probed by Western blot analysis (A). Cells were treated with 1 μM 17-AAG for 1 hour. Protein G-Sepharose beads alone were used to exclude the possibility of nonspecific binding. Treatment with 17-AAG for 1 hour was chosen because of minimal change in total KIT levels during that time (B).

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal