Figure 1. Negative regulation of Src-PTKs by CD45 in B cells. (A) Phosphorylation of Src-PTKs and Syk in BAL-17 cells. BAL-17 and CD45-deficient clone 12 cells were incubated for 5 minutes (–) without or (+) with 20 μg/mL (Fab′)2 fragments of anti-IgM Ab (+); PTKs were then subjected to Western blotting with anti-PY Ab. (B) Fyn activation in BAL-17 and CD45-deficient clone 12 cells. Cells were treated as in panel A; Fyn was then subjected to IVK assays with enolase as a substrate and to blotting with anti-Fyn Ab. (C) Phosphorylation and activation of Lyn in splenic B cells. B cells from B6 and CD45 KO mice were treated as in panel A, after which Lyn was subjected to IVK assays using enolase and to Western blotting with anti-PY and anti-Lyn Abs.
Figure 1.

Negative regulation of Src-PTKs by CD45 in B cells. (A) Phosphorylation of Src-PTKs and Syk in BAL-17 cells. BAL-17 and CD45-deficient clone 12 cells were incubated for 5 minutes (–) without or (+) with 20 μg/mL (Fab′)2 fragments of anti-IgM Ab (+); PTKs were then subjected to Western blotting with anti-PY Ab. (B) Fyn activation in BAL-17 and CD45-deficient clone 12 cells. Cells were treated as in panel A; Fyn was then subjected to IVK assays with enolase as a substrate and to blotting with anti-Fyn Ab. (C) Phosphorylation and activation of Lyn in splenic B cells. B cells from B6 and CD45 KO mice were treated as in panel A, after which Lyn was subjected to IVK assays using enolase and to Western blotting with anti-PY and anti-Lyn Abs.

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