Figure 1. Western blot analysis on tyrosine phosphorylation of FLT3 and STAT5 in leukemia cell lines with or without D835 mutations. Lysates of leukemia cell lines with (lanes 1-2) or without (lanes 3-5) FLT3-D835 mutations were separated, blotted, and stained with antibodies against FLT3, phosphotyrosine (after immunoprecipitation by anti-FLT3), STAT5, phospho-STAT5, and α-tubulin, respectively. Lanes 1, 3, and 4 were B-precursor cell lines with MLL rearrangement; lane 2 was a monocytoid cell line with MLL rearrangement; lane 5 was a T-lymphoid cell line.
Figure 1.

Western blot analysis on tyrosine phosphorylation of FLT3 and STAT5 in leukemia cell lines with or without D835 mutations. Lysates of leukemia cell lines with (lanes 1-2) or without (lanes 3-5) FLT3-D835 mutations were separated, blotted, and stained with antibodies against FLT3, phosphotyrosine (after immunoprecipitation by anti-FLT3), STAT5, phospho-STAT5, and α-tubulin, respectively. Lanes 1, 3, and 4 were B-precursor cell lines with MLL rearrangement; lane 2 was a monocytoid cell line with MLL rearrangement; lane 5 was a T-lymphoid cell line.

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