Fig. 6. Regulation of STAT5 target genes during eosinophil differentiation. / (A) Protein lysates were prepared from CD34+ cells differentiated toward eosinophils for 3, 7, 10, 14, and 17 days. Western blot analysis was performed with antibodies against Bcl-2 (top panel) and p21WAF/Cip1 (middle panel) and as a control for equal loading with an antibody against β-actin (bottom panel). The experiment shown is representative of 3 independent experiments. (B) CD34+ cells were transduced with eGFP, STAT5a, or STAT5aΔ750. After 7, 14, and 17 days of differentiation, eGFP-positive cells were sorted, and protein lysates were prepared and analyzed by Western blotting.
Fig. 6.

Regulation of STAT5 target genes during eosinophil differentiation.

(A) Protein lysates were prepared from CD34+ cells differentiated toward eosinophils for 3, 7, 10, 14, and 17 days. Western blot analysis was performed with antibodies against Bcl-2 (top panel) and p21WAF/Cip1 (middle panel) and as a control for equal loading with an antibody against β-actin (bottom panel). The experiment shown is representative of 3 independent experiments. (B) CD34+ cells were transduced with eGFP, STAT5a, or STAT5aΔ750. After 7, 14, and 17 days of differentiation, eGFP-positive cells were sorted, and protein lysates were prepared and analyzed by Western blotting.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal