Fig. 5. Determination of residues at positions P4 and P9 of HIENFSDIDMGE as critical residues for HLA-DQ5 HY CTL recognition. / Irradiated HLA-DQ5 female EBV cells were incubated with various concentrations of the indicated peptides for 2 hours. After washing, HLA-DQ5 HY CTLs were added; 16 hours later, supernatant was harvested and IFN-γ content was measured. IFN-γ release by HLA-DQ5 HY CTL was completely abrogated when the amino acid at P4 or P9 was substituted by the X-homolog residue. In contrast, substitution of the isoleucin residue at P8 by the DBX-derived valine resulted in an even enhanced cytokine release by the specific CTL clone.
Fig. 5.

Determination of residues at positions P4 and P9 of HIENFSDIDMGE as critical residues for HLA-DQ5 HY CTL recognition.

Irradiated HLA-DQ5 female EBV cells were incubated with various concentrations of the indicated peptides for 2 hours. After washing, HLA-DQ5 HY CTLs were added; 16 hours later, supernatant was harvested and IFN-γ content was measured. IFN-γ release by HLA-DQ5 HY CTL was completely abrogated when the amino acid at P4 or P9 was substituted by the X-homolog residue. In contrast, substitution of the isoleucin residue at P8 by the DBX-derived valine resulted in an even enhanced cytokine release by the specific CTL clone.

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