Fig. 7.
Effect of PI-3 kinase inhibitors on LIBS6-induced thromboxane A2 generation.
Platelets activated with Fab fragments of the LIBS6 antibody after pretreatment with wortmannin (20 nM) or LY294002 (10 μM) at 37°C with stirring for 5 minutes. Fibrinogen was added at the end of the incubation, and stirring was continued for an additional 3 minutes. The reaction was stopped by snap-freezing, and thromboxane B2was estimated as described in Figure 1.
