Fig. 4. DMT1 isoform II is expressed in the spleen of PHZ-treated mice. / (A) Schematic representation of the 2 DMT1 mRNAs (IRE-containing and non–IRE-containing form generated by alternative splicing) and corresponding proteins (isoform I and II, respectively). The 2 isoforms show distinct 3′ UTR and encode polypeptides with distinct carboxy termini (hatched boxes). The DMT1-NT antibody recognizes the N-terminus of both isoforms I and II (solid boxes), whereas the DMT1-CT antibody was raised against the carboxy-terminal extremity of isoform II. (B) Microsomal spleen fractions (120 μg) from control (−) or PHZ-treated (+) mice were analyzed by immunoblotting with the DMT1-CT antiserum. Membrane proteins (5 μg) from CHO cells or CHO transfectant expressing either a cMyc-tagged DMT1 isoform II (CHO-DMT1) or a cMyc-tagged mNramp1 (CHO-Nramp1) were included as controls. The positions and sizes (in kilodaltons) of molecular mass markers are indicted on the right.
Fig. 4.

DMT1 isoform II is expressed in the spleen of PHZ-treated mice.

(A) Schematic representation of the 2 DMT1 mRNAs (IRE-containing and non–IRE-containing form generated by alternative splicing) and corresponding proteins (isoform I and II, respectively). The 2 isoforms show distinct 3′ UTR and encode polypeptides with distinct carboxy termini (hatched boxes). The DMT1-NT antibody recognizes the N-terminus of both isoforms I and II (solid boxes), whereas the DMT1-CT antibody was raised against the carboxy-terminal extremity of isoform II. (B) Microsomal spleen fractions (120 μg) from control (−) or PHZ-treated (+) mice were analyzed by immunoblotting with the DMT1-CT antiserum. Membrane proteins (5 μg) from CHO cells or CHO transfectant expressing either a cMyc-tagged DMT1 isoform II (CHO-DMT1) or a cMyc-tagged mNramp1 (CHO-Nramp1) were included as controls. The positions and sizes (in kilodaltons) of molecular mass markers are indicted on the right.

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