Fig. 2. Cytokine mRNA expression in Tax transgenic mouse tumors. / Total RNA (10 μg) was extracted from fresh NT (lane 1) and Tax transgenic mouse tissues (lanes 2 and 3) or from the tumor-derived F8 LGL cell line (lane 4) and used in RNase protection assays using a multiprobe cytokine system. The L32 and GAPDH probes were used to demonstrate equivalent RNA levels in each sample. A total of 4 primary Tax-induced tumors and 5 Tax spleens were analyzed, and a representative gel is shown.
Fig. 2.

Cytokine mRNA expression in Tax transgenic mouse tumors.

Total RNA (10 μg) was extracted from fresh NT (lane 1) and Tax transgenic mouse tissues (lanes 2 and 3) or from the tumor-derived F8 LGL cell line (lane 4) and used in RNase protection assays using a multiprobe cytokine system. The L32 and GAPDH probes were used to demonstrate equivalent RNA levels in each sample. A total of 4 primary Tax-induced tumors and 5 Tax spleens were analyzed, and a representative gel is shown.

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