Fig. 1. Correlation of flow cytometry and Southern blot analysis to detect human engraftment in NOD/SCID mice. / Data are from 2 representative animals transplanted with 0.6 × 106 human CD34+ PBSCs and killed 8 weeks after transplantation. (A) Southern blot analysis of the BM and spleen of 2 mice. Human:mouse DNA controls are given as percent human DNA. (B) BM and spleen isotype controls for FITC (x-axis) and PE (Y-axis). (C) Evaluation of human cells labeled with antihuman CD19-PE and CD45-FITC monoclonal antibodies in the BM and spleen. The percentage of human cells is indicated.
Fig. 1.

Correlation of flow cytometry and Southern blot analysis to detect human engraftment in NOD/SCID mice.

Data are from 2 representative animals transplanted with 0.6 × 106 human CD34+ PBSCs and killed 8 weeks after transplantation. (A) Southern blot analysis of the BM and spleen of 2 mice. Human:mouse DNA controls are given as percent human DNA. (B) BM and spleen isotype controls for FITC (x-axis) and PE (Y-axis). (C) Evaluation of human cells labeled with antihuman CD19-PE and CD45-FITC monoclonal antibodies in the BM and spleen. The percentage of human cells is indicated.

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