Fig. 2. Synergy between Ca++-and TPA-dependent induction of KSHV lytic protein expression. / Viable BCBL-1 cells stimulated with 1 μg/mL ionomycin, 3 nM TPA, or both were examined for PF-8 (A) or gpK8.1 (B) expression. Unstimulated cells (○), TPA (●), ionomycin (■), TPA + ionomycin (▪). The dotted line with no symbols represents the sum of the number of positive cells induced by TPA (●) and the number of positive cells induced by ionomycin (■). Data represent mean ± SEM of at least 3 separate experiments.
Fig. 2.

Synergy between Ca++-and TPA-dependent induction of KSHV lytic protein expression.

Viable BCBL-1 cells stimulated with 1 μg/mL ionomycin, 3 nM TPA, or both were examined for PF-8 (A) or gpK8.1 (B) expression. Unstimulated cells (○), TPA (●), ionomycin (■), TPA + ionomycin (▪). The dotted line with no symbols represents the sum of the number of positive cells induced by TPA (●) and the number of positive cells induced by ionomycin (■). Data represent mean ± SEM of at least 3 separate experiments.

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