A truncated mutant form of the FANCG protein translocates to the cell nucleus but fails to function.

The primary FA-G fibroblast line, DF3, was transduced with retroviral constructs encoding the indicated wild-type or mutant forms of the FANCG protein. Following retroviral transduction, the cells were analyzed by immunofluorescence with anti-FANCG antisera or by staining with the DNA-specific dye, DAPI (4,6-diamidino-2-phenylindole). In parallel, the cells were analyzed for correction of MMC sensitivity (data not shown). The wild-type FANCG and FANCG(Q26R) protein corrected the MMC sensitivity of the DF3 cells, but the FANCG 1-428 mutant failed to complement the cells.