Fig. 3.
Fig. 3. Staining for TUNEL and annexin-V before and after culture with or without Ub treatment. / (A) HL-60 and KT-3 cells were cultured under the conditions described in Figure 2. The cells were subjected to staining for TUNEL and annexin-V and analyzed on FACSort. (B) Flow cytometric analyses on caspase-3 activities. Caspase-3 activities were measured by a fluorescence intensity that derives from the caspase-3–cleaved substrate by flow cytometry. A height of right fluorescent peak indicates the degree of caspase-3 activation.

Staining for TUNEL and annexin-V before and after culture with or without Ub treatment.

(A) HL-60 and KT-3 cells were cultured under the conditions described in Figure 2. The cells were subjected to staining for TUNEL and annexin-V and analyzed on FACSort. (B) Flow cytometric analyses on caspase-3 activities. Caspase-3 activities were measured by a fluorescence intensity that derives from the caspase-3–cleaved substrate by flow cytometry. A height of right fluorescent peak indicates the degree of caspase-3 activation.

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