Fig. 1. Expression of MEFV mRNA in different populations of peripheral blood cells. / Total RNAs were isolated from the indicated subsets of leukocytes and subjected to RT-PCR using specific oligonucleotides for MEFV, lactoferrin, and β-actin. Amplification occurred for 30, 25, and 22 cycles, respectively. PCR fragments were separated by agarose gel electrophoresis, transferred to a nylon membrane, and hybridized with a gene-specific internal oligonucleotide labeled with DIG. (PMN = polymorphonuclear cells (neutrophils); Monoc. = monocytes; M = DNA length standard VI: 154, 220/234, 298, 394, 453, 517, 653, 1033 bp, Boehringer Mannheim.)
Fig. 1.

Expression of MEFV mRNA in different populations of peripheral blood cells.

Total RNAs were isolated from the indicated subsets of leukocytes and subjected to RT-PCR using specific oligonucleotides for MEFV, lactoferrin, and β-actin. Amplification occurred for 30, 25, and 22 cycles, respectively. PCR fragments were separated by agarose gel electrophoresis, transferred to a nylon membrane, and hybridized with a gene-specific internal oligonucleotide labeled with DIG. (PMN = polymorphonuclear cells (neutrophils); Monoc. = monocytes; M = DNA length standard VI: 154, 220/234, 298, 394, 453, 517, 653, 1033 bp, Boehringer Mannheim.)

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