![Fig. 5. Autoradiogram showing phosphoproteins generated by protein kinase(s) present in immobilized erythrocyte immunoprecipitates. Sephacryl S-300 column fractions no. 40 through 51, combined and concentrated 6-fold, were incubated with agarose-linked antiphosphotyrosine (PT66) in the presence of low ionic strength buffers. Immobilized immunoprecipitates were incubated with protein kinase buffer augmented with 1 μmol/L [γ-32P]ATP (4 × 107 dpm) and 20 mmol/L magnesium acetate. The 12-, 30-, 36-, 42-, 60-, 72-, and 165-kD phosphoproteins are indicated on the right. Molecular mass markers (6.5 to 204 kD) are on the left.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/94/8/10.1182_blood.v94.8.2778.420k10_2778_2789/7/blod42010005w.jpeg?Expires=1763300404&Signature=aAtfGH1AIPIKHfLazAjnkeCC92GyDbCPfT-2x2c6K6PL1~jW9Y52J5B5LOqA6XBfCH9hkOmDs0desr4nDZKutxRjsURajeg-d3NRPgyguyzZKtgtDodkIz4iUJWAelbN~Gesq1U2qvcjHWwPqHYSlPL7qfyaa6sFiTicbQZVI-Asg8IsGqxYvc9XV0YuwDEkUnEY8NQOJV5FlmASFBgYXRIfPNDPW2VrmzPkoTbVxsbByINQ2n4Q-LJR1s2ExyvskOf2SmpNE9wwzfYTdwZ-GvTFExbjcfnDAi-hPsc7PbTeFOYlstDzrbf-AeQOxBOk~dKMiPBj9z8p2BGDMOsaKA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Fig. 5.
Autoradiogram showing phosphoproteins generated by protein kinase(s) present in immobilized erythrocyte immunoprecipitates. Sephacryl S-300 column fractions no. 40 through 51, combined and concentrated 6-fold, were incubated with agarose-linked antiphosphotyrosine (PT66) in the presence of low ionic strength buffers. Immobilized immunoprecipitates were incubated with protein kinase buffer augmented with 1 μmol/L [γ-32P]ATP (4 × 107 dpm) and 20 mmol/L magnesium acetate. The 12-, 30-, 36-, 42-, 60-, 72-, and 165-kD phosphoproteins are indicated on the right. Molecular mass markers (6.5 to 204 kD) are on the left.
