Fig. 3. Time course of DAF upregulation on HUVECs and DMECs by cytokines. EC monolayers were stimulated for up to 72 hours with plain medium or a combination of TNF- (10 ng/mL) and IFN-γ (500 U/mL). After harvesting, single-cell suspensions were analyzed by flow cytometry for DAF expression using MoAb 5B2. (A) Upregulation of DAF on DMECs and HUVECs after 48 hours of stimulation with a combination of TNF- and IFN-γ: negative control (…), constitutive expression of DAF (—), and TNF- and INF-γ–induced DAF (filled histograms). (B) Kinetics for DAF expression on DMECs and HUVECs following stimulation with TNF- and IFN-γ. The figure is representative of 3 similar experiments on each cell type.
Fig. 3.

Time course of DAF upregulation on HUVECs and DMECs by cytokines. EC monolayers were stimulated for up to 72 hours with plain medium or a combination of TNF- (10 ng/mL) and IFN-γ (500 U/mL). After harvesting, single-cell suspensions were analyzed by flow cytometry for DAF expression using MoAb 5B2. (A) Upregulation of DAF on DMECs and HUVECs after 48 hours of stimulation with a combination of TNF- and IFN-γ: negative control (…), constitutive expression of DAF (—), and TNF- and INF-γ–induced DAF (filled histograms). (B) Kinetics for DAF expression on DMECs and HUVECs following stimulation with TNF- and IFN-γ. The figure is representative of 3 similar experiments on each cell type.

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