Fig. 2. CXCR4 expression on megakaryocytes and platelets. (A, B) Representative data of flow cytometric analysis of CD41a and CXCR4 on megakaryocytes grown in culture are shown. Cord blood CD34+ cells were cultured for 6 days in the presence of a combination of SCF and PEG-rhuMGDF. Cells were stained using an anti-CD41a MoAb in combination with either an isotype control antibody (A) or the anti-CXCR4 MoAb (B). (C) CXCR4 expression on freshly isolated megakaryocytes after percoll enrichment. (D) Platelets, depleted in leukocytes, were labeled using a similar procedure. Labeling with control MoAb is shown by the thin line. The thick line shows the staining with the anti-CXCR4 MoAb.
Fig. 2.

CXCR4 expression on megakaryocytes and platelets. (A, B) Representative data of flow cytometric analysis of CD41a and CXCR4 on megakaryocytes grown in culture are shown. Cord blood CD34+ cells were cultured for 6 days in the presence of a combination of SCF and PEG-rhuMGDF. Cells were stained using an anti-CD41a MoAb in combination with either an isotype control antibody (A) or the anti-CXCR4 MoAb (B). (C) CXCR4 expression on freshly isolated megakaryocytes after percoll enrichment. (D) Platelets, depleted in leukocytes, were labeled using a similar procedure. Labeling with control MoAb is shown by the thin line. The thick line shows the staining with the anti-CXCR4 MoAb.

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