Fig. 3. Mutational alterations in the DNA binding and heterodimerization activities of the Runt domain. (A) The partial AML1 proteins indicated above the panel were produced in E coli and subjected to EMSA. Symbols + and − signify the presence and absence of the β2 subunit, respectively. (B) The indicated partial AML1 proteins were subjected to an affinity column assay. M, molecular weight marker; β, PEBP2β2; A, input AML1 protein; W, unbound proteins in washed fractions; E, bound proteins eluted at 250 mmol/L imidazole. The bands marked with open triangles indicate the β subunits associated with AML1 proteins.
Fig. 3.

Mutational alterations in the DNA binding and heterodimerization activities of the Runt domain. (A) The partial AML1 proteins indicated above the panel were produced in E coli and subjected to EMSA. Symbols + and − signify the presence and absence of the β2 subunit, respectively. (B) The indicated partial AML1 proteins were subjected to an affinity column assay. M, molecular weight marker; β, PEBP2β2; A, input AML1 protein; W, unbound proteins in washed fractions; E, bound proteins eluted at 250 mmol/L imidazole. The bands marked with open triangles indicate the β subunits associated with AML1 proteins.

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