![Fig. 1. Specificity of the newly established MoAbs HSL11, HSL96, and HSL2 for human λ5, VpreB, and pre-BCR, respectively. A pre–B-cell line NALM6 (A), a pro–B-cell line RS4;11 (B), and X63.Ag8-653 cells transfected with either the λ5 or VpreB gene (C) were biosynthetically labeled with [35S]-methionine for 4 hours and lysed with 1% NP-40 lysis buffer. Detergent-soluble lysates were incubated with indicated MoAbs, and immunoprecipitates were analyzed by 13% SDS polyacrylamide gel electrophoresis under reducing conditions. The positions of μH, λ5, and VpreB are indicated by arrows, and a 16-kD band precipitated by HSL11 together with λ5 is indicated by an arrow head.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/92/11/10.1182_blood.v92.11.4317/5/blod42343001w.jpeg?Expires=1763556674&Signature=VoR1Fv3sQIOs~6~xV3iuFv29VuNuwprC3S-HIcS8MxyjQb6NR~vNH2-R7yyVlOls02j2~ern-CLrkQ-rpxomAcpQiGXfo2e8LZX52IR8cYPNDEoDSpl~jwY5agf3YE6HhKpbjquqDD2S7N-TT9cUo3Sd9xNMnaezHh8zZyhciRxl~SKzoRrOK7TWx2qH118s9mcHdV31X4goaoHujw5UFbGh0AVgqYXQJ~TsdAVSkeKjpJYJf5rL2Di01slted04k~jIZ3NFP-jnTSGafdppkTVlPwfBrfuto6XfPWrnIeC8IRC0VbEis7XXQI4LXh4TELs6dTQeidf9Txy1NmT5wQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Fig. 1.
Specificity of the newly established MoAbs HSL11, HSL96, and HSL2 for human λ5, VpreB, and pre-BCR, respectively. A pre–B-cell line NALM6 (A), a pro–B-cell line RS4;11 (B), and X63.Ag8-653 cells transfected with either the λ5 or VpreB gene (C) were biosynthetically labeled with [35S]-methionine for 4 hours and lysed with 1% NP-40 lysis buffer. Detergent-soluble lysates were incubated with indicated MoAbs, and immunoprecipitates were analyzed by 13% SDS polyacrylamide gel electrophoresis under reducing conditions. The positions of μH, λ5, and VpreB are indicated by arrows, and a 16-kD band precipitated by HSL11 together with λ5 is indicated by an arrow head.
