Fig. 6. Detection of proviral genome in secondary colonies derived from engrafted NOD/SCID mice. Secondary colonies were established with cells obtained from the BM of engrafted NOD/SCID animals 6 weeks after injection. The presence of LNSN proviral DNA in hematopoietic colonies derived from three mice injected with CB#1 (A), one mouse injected with CB#3 (B), and one mouse injected with CB#5 (C) was assessed by a sensitive LNGFR-specific PCR. The 425-bp–specific LNGFR product was detected by Southern blotting. Amplification of a sequence from the human mannose binding protein (MBP) gene was used to control for the presence of DNA.63
Fig. 6.

Detection of proviral genome in secondary colonies derived from engrafted NOD/SCID mice. Secondary colonies were established with cells obtained from the BM of engrafted NOD/SCID animals 6 weeks after injection. The presence of LNSN proviral DNA in hematopoietic colonies derived from three mice injected with CB#1 (A), one mouse injected with CB#3 (B), and one mouse injected with CB#5 (C) was assessed by a sensitive LNGFR-specific PCR. The 425-bp–specific LNGFR product was detected by Southern blotting. Amplification of a sequence from the human mannose binding protein (MBP) gene was used to control for the presence of DNA.63 

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