Fig. 1. The effect of HNE on F.IX and F.IXa as determined by aPTT clotting assay. Purified F.IX or F.IXa (4 μmol/L of each) was incubated either alone (control) or with HNE (400 nmol/L) in HBS/Ca at 37°C. After various times, two simultaneous aliquots of the same reaction mixtures were withdrawn and assayed for either aPTT clotting activity after the addition of 10 μmol/L AAPV-CMK (A and B) or analyzed by reducing SDS-PAGE (see Fig 2 below). The relative F.IX and F.IXa aPTT clotting activity (normalized to the time = 0 sample in each case) versus incubation time with (•) or without (○) added HNE is plotted in (A) and (B), respectively.
Fig. 1.

The effect of HNE on F.IX and F.IXa as determined by aPTT clotting assay. Purified F.IX or F.IXa (4 μmol/L of each) was incubated either alone (control) or with HNE (400 nmol/L) in HBS/Ca at 37°C. After various times, two simultaneous aliquots of the same reaction mixtures were withdrawn and assayed for either aPTT clotting activity after the addition of 10 μmol/L AAPV-CMK (A and B) or analyzed by reducing SDS-PAGE (see Fig 2 below). The relative F.IX and F.IXa aPTT clotting activity (normalized to the time = 0 sample in each case) versus incubation time with (•) or without (○) added HNE is plotted in (A) and (B), respectively.

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