Fig. 3. Effect of ATRA on TF, TNF-α, and IL-8 mRNA expression in LPS-stimulated THP-1 cells. Total RNA was extracted from THP-1 cells exposed to LPS (10 μg/mL) for 2 hours with or without a 30-minute pretreatment with ATRA (1 × 10−5 mol/L). Control samples were incubated with ATRA alone or without LPS or ATRA. Various mRNA levels were determined by Northern blot analysis using radiolabeled human cDNA probes. Membranes were reprobed with a G3PDH cDNA probe to assess RNA loading. The positions of TF (A), TNF-α (B), and IL-8 (C) mRNAs are indicated. Similar results were observed in one or more independent experiments.
Fig. 3.

Effect of ATRA on TF, TNF-α, and IL-8 mRNA expression in LPS-stimulated THP-1 cells. Total RNA was extracted from THP-1 cells exposed to LPS (10 μg/mL) for 2 hours with or without a 30-minute pretreatment with ATRA (1 × 10−5 mol/L). Control samples were incubated with ATRA alone or without LPS or ATRA. Various mRNA levels were determined by Northern blot analysis using radiolabeled human cDNA probes. Membranes were reprobed with a G3PDH cDNA probe to assess RNA loading. The positions of TF (A), TNF-α (B), and IL-8 (C) mRNAs are indicated. Similar results were observed in one or more independent experiments.

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