Fig. 2.
Localization of sPLA2 in human eosinophils isolated from peripheral blood. Ultrathin cryosections were incubated with MoAb 10B2, rabbit antimouse IgG and goat antirabbit IgG conjugated to 10-nm gold particles. (A) Overview of an eosinophil showing labeling mainly on the matrix of specific granules, although these granules do show heterogeneity. Nucleus (n); bar = 500 nm. (B) Detail of the cytoplasm showing dense labeling in some granules (closed arrows) and absence of label in others (open arrows). Bar = 200 nm.
