Expression of G1-phase cyclins by qPCR. The expression of (A) CCND2, (B) CCND3, (C) CCNE1, and (D) CCNE2 was represented according to 4 categories: MCL positive or negative for the CCND2 break-apart FISH test (CCND2 BAP⁺ and CCND2 BAP⁻, respectively), CLL, and nonneoplastic lymphoid samples (normal). Colored dots highlight cases with overexpression of CCND2 (dark blue), CCND3 (light blue), CCNE1/CCNE2 (orange). The overexpression cutoff values were calculated as the mean plus 3 standard deviations in nonrearranged cases: 15.1 for CCND2, 6.2 for CCND3, 3 for CCNE1, and 2.4 for CCNE2. The y-axis of the CCND2 graph has been transformed for the expression values above 50 (dashed arrow).