![Figure 4. The protein antigen-specific T-cell stimulatory capacity of SP37A3 cells correlates with their state of maturation. OVA-specific T-cell stimulatory capacity of differentially conditioned BM-DCs (left panels) and SP37A3 cells (right panels) was assessed by determining proliferation of OT-II T cells. DCs were differentiated as described (Figures 1,3) and were pulsed for 3 hours with 10 μg/mL OVA323-339 peptide or medium and were irradiated. (A) Titrated numbers of DCs were cocultured with 5 × 104 OT-II T cells for 3 days in triplicate cultures. T-cell proliferation was assessed as uptake of [3H] thymidine for the final 16 hours of culture. Data represent mean ± SEM of triplicate cultures and are representative of 3 independent experiments. (B) DCs (3 × 103) were cocultured with CFSE-labeled T cells (5 × 104) for 3 days, and T-cell proliferation was determined as relative CFSE staining intensity by flow cytometry. Graphs are representative of 3 independent experiments. *P < .05 compared with immature DCs and DCs matured in the presence of DEX (10−5 M); +P < .05 compared with alternatively activated DCs (10−5 M).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/109/9/10.1182_blood-2006-07-035576/4/zh80090700400004.jpeg?Expires=1769561349&Signature=P89Xxc5bmY5Hex9x3UhKsZxhoo5P4inryDlVrKNe-lZmmg~3dQuUD5KG8t76F0Ej-Y1hc1ssEKiPggAIzPo4PFaDGpCp7vEmPJuWwVaF96ERJpbE6gTwsfrxrDKX1p-6VgDAnp~QUIBn-jzlK1EPBl65i13nPjfQ~XenhsfBDeW6lQq0VHU963uHobKdeKQIG0Mp~8KKTgo8eAbnIUaksfG8AVcQsXe8vpNwRuMmx~o7wvJtOii37k~0RKEJHbFMc6sP9M83tGl~W30xX~cvgG5B22geGC~kpDTMWxs6pTekSCNC-2PTHj2PL1-AXR99ynLiE9LCIkbhzhgbJ6Xgvw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
The protein antigen-specific T-cell stimulatory capacity of SP37A3 cells correlates with their state of maturation. OVA-specific T-cell stimulatory capacity of differentially conditioned BM-DCs (left panels) and SP37A3 cells (right panels) was assessed by determining proliferation of OT-II T cells. DCs were differentiated as described (Figures 1,3) and were pulsed for 3 hours with 10 μg/mL OVA323-339 peptide or medium and were irradiated. (A) Titrated numbers of DCs were cocultured with 5 × 104 OT-II T cells for 3 days in triplicate cultures. T-cell proliferation was assessed as uptake of [3H] thymidine for the final 16 hours of culture. Data represent mean ± SEM of triplicate cultures and are representative of 3 independent experiments. (B) DCs (3 × 103) were cocultured with CFSE-labeled T cells (5 × 104) for 3 days, and T-cell proliferation was determined as relative CFSE staining intensity by flow cytometry. Graphs are representative of 3 independent experiments. *P < .05 compared with immature DCs and DCs matured in the presence of DEX (10−5 M); +P < .05 compared with alternatively activated DCs (10−5 M).
