Infarct volumes and functional outcomes 24 hours after transient middle cerebral artery occlusion in WT mice, VWF^−/− mice, and VWF^−/− mice after reconstitution with plasma VWF (gene transfer). (A top) Representative 2,3,5-TTC stains of 3 corresponding coronal brain sections of the 3 groups at day 1 after transient middle cerebral artery occlusion (tMCAO). (Bottom) Brain infarct volumes of the 3 groups as measured by planimetry at day 1 after tMCAO (n = 10 per group). (B) Neurologic Bederson score (left) and grip test score (right) of the 3 groups as assessed at day 1 after tMCAO (n = 10 per group). (C) Hematoxylin and eosin (H&E)–stained sections of corresponding territories in the ischemic hemispheres of wild-type (WT) and von Willebrand factor (VWF)^−/− mice. Infarcts are restricted to the basal ganglia in VWF^−/− mice but consistently include the neocortex in WT controls. Bar represents 100 μm or 20 μm (enlarged picture), **P < .01, *P < .05; unpaired 2-tailed Student t test compared with WT mice.