A coordinate profile of Bcl-2 expression, p53 expression, and p53 phosphorylation is observed in primary AML samples. (A) Expression of Bcl-2, p53, p-p53-ser15, p-p53-ser20, p-p53-ser37, p-p53-ser46, and p-p53-ser392 was measured for all 30 patient samples. Three clusters of p-p53 patterns were detected: low-phospho-p53; phosphorylation at serine 20 or 37; or phosphorylation at serine 15, 46, or 392. (B) Correlations between p53 phosphorylation and Bcl-2 expression were analyzed by linear regression. Data for each of 30 AML patient samples were plotted in 2 dimensions to determine dependence. Bcl-2 was significantly correlated with p53 phosphorylation at serines 15, 46, and 392.