NK cell development is altered by AML cells. (A) Graphical illustration of NK cell development coculture in vitro. (B) Postculture flow cytometry analysis of CD94⁺ mature NK cells in the presence of AML vs control. (C) Stage 3 NK cells were cocultured via transwell with MV411 AML cells with and without treatment with the AHR inhibitor, CH223191 (CH); n = 14 independent donors. Stage 3 (S3) Lin⁻CD34⁻CD117⁺CD94⁻NKp80⁻. (D) Post-coculture, IFN-γ was evaluated following phorbol 12-myristate 13-acetate/ionomycin stimulation; n = 5 independent donors. Student t test, *P < .05; **P < .01; ***P < .001; ****P < .0001. Error bars indicate SD.