qPCR. β-globin (Hbb) expression of high allele strains (color-coded) relative to the corresponding low allele strain in (A) spleen and (B) bone marrow (BM) in crosses detecting Chcmq3 (Table 2). qPCR primers (supplemental Table 1) exactly matched sequences in β^S, β^T, β^dmajor, and β^dminor. Bone marrow (C) and liver (D) Trf expression in crosses in which Chcmq2 was detected. Because strain A was the high allele in 2 different crosses, the specific cross is indicated directly on the bar graph. *Expression in high and low allele strains differ significantly (P ≤ .028).