Stereomicroscope analysis of FL homing of enriched HSCs following IUHCT. E14 BALB/c fetal mice were injected intravenously with 1 × 105 B6GFP-enriched HSCs without (A,C) or with (B,D) CD26 inhibition with 5 mM Diprotin A. Fetal livers of recipient mice were harvested at 4 hours (A-B) and 48 hours (C-D) after injection and assessed for the presence of GFP donor cells by fluorescence stereomicropscope. All images were obtained with a Leica MZ16FA stereoscopic fluorescent microscope (Leica Microsystems, Bannockburn, IL) at an exposure time of 800 milliseconds and 25× microscope (1×/0.25 Leica Planapochromatic objective). Images were photographed with a Leica DFC350FX camera, acquired by Openlab 4.0 (Improvision, Lexington, MA) image acquisition software, and combined into a single image using Photoshop 7.0 (Adobe Systems, San Jose, CA) without image enhancement.
