Fig. 2. Relative expression levels of Hif1a exon I.1 and exon I.2-containing mRNA isoforms in various mouse tissues. RNase protection assays using each 50 μg total RNA and exon I.1 and I.2-specific antisense probes. A total of 2 μg total RNA was used for the β-actin control reactions. Besides the sole protected 113-bp fragment in Hepa1 cells, a second mRNA species appeared in mouse tissues probably exceeding the 5′ end of the exon I.1 probe (134 nt).
Fig. 2.

Relative expression levels of Hif1a exon I.1 and exon I.2-containing mRNA isoforms in various mouse tissues. RNase protection assays using each 50 μg total RNA and exon I.1 and I.2-specific antisense probes. A total of 2 μg total RNA was used for the β-actin control reactions. Besides the sole protected 113-bp fragment in Hepa1 cells, a second mRNA species appeared in mouse tissues probably exceeding the 5′ end of the exon I.1 probe (134 nt).

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