Fig. 8. Activation of p21ras by cytokines and chemoattractants in human eosinophils and neutrophils. In (A) eosinophils (6 × 106) or (B) neutrophils (107) were stimulated as indicated (IL-5, 10−10 mol/L; PAF, 10−6mol/L; fMLP, 10−6 mol/L; GM-CSF, 10−10mol/L) before lysis and incubation with GST-Raf1(RBD) to bind GTP-bound p21ras as described in Materials and Methods. Samples were analyzed by SDS-PAGE and immunoblotting with a MoAb against p21ras. The position of GTP-bound p21ras is marked by an arrowhead. This is representative of three individual experiments.
Fig. 8.

Activation of p21ras by cytokines and chemoattractants in human eosinophils and neutrophils. In (A) eosinophils (6 × 106) or (B) neutrophils (107) were stimulated as indicated (IL-5, 10−10 mol/L; PAF, 10−6mol/L; fMLP, 10−6 mol/L; GM-CSF, 10−10mol/L) before lysis and incubation with GST-Raf1(RBD) to bind GTP-bound p21ras as described in Materials and Methods. Samples were analyzed by SDS-PAGE and immunoblotting with a MoAb against p21ras. The position of GTP-bound p21ras is marked by an arrowhead. This is representative of three individual experiments.

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