Fig. 5. E10.5 Yolk sac hematopoiesis. (A and B) Dark-field illumination of Mll +/+ (A) or Mll −/− (B) colonies from dissociated yolk sacs cultured for 9 days in methylcellulose media containing IL3, IL-6, and stem cell factor (original magnification × 3.7). (C and D) Colonies with CFU-E morphology in Mll +/+ (C) or Mll −/− (D) cultures grown for 5 days in methylcellulose media containing recombinant erythropoietin (original magnification × 134). (E and F ) Colonies with CFU-GM morphology in Mll +/+ (E) or Mll −/− (F ) cultures grown for 5 days in methylcellulose media containing recombinant IL3, IL6, and stem cell factor. The Mll −/− colony is small and poorly formed (original magnification × 67). (G and H) Maturing myeloid elements and macrophages in Wright-Giemsa–stained smear preparations from Mll +/+ (G) and Mll −/− (H) cultures after 10 days in culture (original magnification × 810).
Fig. 5.

E10.5 Yolk sac hematopoiesis. (A and B) Dark-field illumination of Mll +/+ (A) or Mll −/− (B) colonies from dissociated yolk sacs cultured for 9 days in methylcellulose media containing IL3, IL-6, and stem cell factor (original magnification × 3.7). (C and D) Colonies with CFU-E morphology in Mll +/+ (C) or Mll −/− (D) cultures grown for 5 days in methylcellulose media containing recombinant erythropoietin (original magnification × 134). (E and F ) Colonies with CFU-GM morphology in Mll +/+ (E) or Mll −/− (F ) cultures grown for 5 days in methylcellulose media containing recombinant IL3, IL6, and stem cell factor. The Mll −/− colony is small and poorly formed (original magnification × 67). (G and H) Maturing myeloid elements and macrophages in Wright-Giemsa–stained smear preparations from Mll +/+ (G) and Mll −/− (H) cultures after 10 days in culture (original magnification × 810).

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