![Fig. 6. Colony formation of sorted bone marrow cells. Fractionated bone marrow cells were cultured in methylcellulose medium containing the following factors: (A) IL-3 + SCF + Epo; (B) IL-3 + SCF + Epo (FULL), IL-3 + Epo [SCF(−)], or IL-3 + SCF [Epo(−)]. Five thousand cells of each fraction (HTK−c-Kit+ and HTK+c-Kit+) were plated. The numbers of colonies were counted on days 7 and 14. Bars show the mean of duplicate culture dishes. E, GM, or MIX indicate colonies derived from CFU-E/BFU-E, CFU-GM, or CFU-MIX, respectively. Results are representative of at least seven independent experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/8/10.1182_blood.v89.8.2757/4/bl_0030f6.jpeg?Expires=1763999367&Signature=wSv62-6eBVJmjGWknfwf86bvKf5LCyn7aI2zTE5TFqvx3AYfZGEOsxd9wiJK~u2LItSxXnfCPIrOQdBBfMclIcHw1mA79qHzxo57DiaK8iljlA4aDy5lH60MSfXFjCe-E9OwanDj2hwb9hLCtOGhhSQtF2wF1Rj6Y65OmOUjWFz1IvgOhZ2D0Uh7NYAvOP9OfTR2OnBBf0uRsDeI4Peo0DTHxAVyLz7qKheZCXLj4iflacQpjtiuzXZof3WJbhbmekpyoQqD4Fc7b8WnR9CF2YgkQEvghSgNmoupT6GKCYe7WAuWpG8MXS88252r0ZUT3QcYnUMxJOyEzSVe~SIMqw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Fig. 6.
Colony formation of sorted bone marrow cells. Fractionated bone marrow cells were cultured in methylcellulose medium containing the following factors: (A) IL-3 + SCF + Epo; (B) IL-3 + SCF + Epo (FULL), IL-3 + Epo [SCF(−)], or IL-3 + SCF [Epo(−)]. Five thousand cells of each fraction (HTK−c-Kit+ and HTK+c-Kit+) were plated. The numbers of colonies were counted on days 7 and 14. Bars show the mean of duplicate culture dishes. E, GM, or MIX indicate colonies derived from CFU-E/BFU-E, CFU-GM, or CFU-MIX, respectively. Results are representative of at least seven independent experiments.
