Fig. 2. HOXA9 targeting and genotypic analysis. (A) Normal HOXA9 genomic locus is shown on top and the targeted locus on the bottom. Arrowheads marked a-9#1, a-9#2, and neo denote primer sites for genotyping by PCR. (B) PCR products from tail or toe DNA, amplified using the 3 primers shown in (A). Amplification of the wild-type allele with primers a-9#1 and a-9#2 results in the lower 123-nt band, whereas amplification of the targeted allele with primers a-9#1 and neo results in the upper 200-nt band (arrows). The gel shows results from normal (lanes 1, 2, 3, and 8), HOXA9+/− (lane 4), and HOXA9−/− (lanes 5 and 6) animals. Lane 7 is a control (C) lane with no DNA.
Fig. 2.

HOXA9 targeting and genotypic analysis. (A) Normal HOXA9 genomic locus is shown on top and the targeted locus on the bottom. Arrowheads marked a-9#1, a-9#2, and neo denote primer sites for genotyping by PCR. (B) PCR products from tail or toe DNA, amplified using the 3 primers shown in (A). Amplification of the wild-type allele with primers a-9#1 and a-9#2 results in the lower 123-nt band, whereas amplification of the targeted allele with primers a-9#1 and neo results in the upper 200-nt band (arrows). The gel shows results from normal (lanes 1, 2, 3, and 8), HOXA9+/− (lane 4), and HOXA9−/− (lanes 5 and 6) animals. Lane 7 is a control (C) lane with no DNA.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal