Figure 6. NICD overexpression in ECs rescues the EHT defects in hif-1α and hif-2α morphants. (A-H) Brightfield images of WISH for runx1/cmyb expression in (A-B) 36 hpf control MO-injected WT and Tg(hsp70:GAL4;UAS:NICD) embryos at 28°C, (C-D) control MO-injected WT and Tg(hsp70:GAL4;UAS:NICD) embryos after heat shock, (E-F) hif-1α MO-injected WT and Tg(hsp70:GAL4;UAS:NICD) embryos after heat shock, and (G-H) hif-2α MO-injected WT and Tg(hsp70:GAL4;UAS:NICD) embryos after heat shock. (I) Quantification of runx1/cmyb WISH results (A-H), showing the percentages of embryos with WT, increased expression, and decreased expression in each condition. (J-O) Brightfield images of WISH for runx1/cmyb expression in (J-K) 36 hpf control MO-injected WT and Tg(fli1a:GAL4;UAS:NICD) embryos, (L-M) hif-1α MO-injected WT and Tg(fli1a:GAL4;UAS:NICD) embryos, and (N-O) hif-2α MO-injected WT and Tg(fli1a:GAL4;UAS:NICD) embryos (lateral views). (P) Quantification of runx1/cmyb WISH results (J-O), showing the percentages of embryos with WT, increased expression, and decreased expression in each condition. Scale bar, 100 μm.
Figure 6.

NICD overexpression in ECs rescues the EHT defects in hif-1α and hif-2α morphants. (A-H) Brightfield images of WISH for runx1/cmyb expression in (A-B) 36 hpf control MO-injected WT and Tg(hsp70:GAL4;UAS:NICD) embryos at 28°C, (C-D) control MO-injected WT and Tg(hsp70:GAL4;UAS:NICD) embryos after heat shock, (E-F) hif-1α MO-injected WT and Tg(hsp70:GAL4;UAS:NICD) embryos after heat shock, and (G-H) hif-2α MO-injected WT and Tg(hsp70:GAL4;UAS:NICD) embryos after heat shock. (I) Quantification of runx1/cmyb WISH results (A-H), showing the percentages of embryos with WT, increased expression, and decreased expression in each condition. (J-O) Brightfield images of WISH for runx1/cmyb expression in (J-K) 36 hpf control MO-injected WT and Tg(fli1a:GAL4;UAS:NICD) embryos, (L-M) hif-1α MO-injected WT and Tg(fli1a:GAL4;UAS:NICD) embryos, and (N-O) hif-2α MO-injected WT and Tg(fli1a:GAL4;UAS:NICD) embryos (lateral views). (P) Quantification of runx1/cmyb WISH results (J-O), showing the percentages of embryos with WT, increased expression, and decreased expression in each condition. Scale bar, 100 μm.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal