18q21 gain or BCL2 translocation is associated with BCL2 protein expression. (A) BCL2 IHC was performed and scored on adult-mBL cases with no evidence of BCL2 gain/translocation based on clinical data, NGS, and SNP array (n = 7) and cases with BCL2 gain/translocation (n = 6). Bars represent the mean expression score and error bars represent 1 standard deviation. (B) Example of BCL2-positive and BCL2-negative staining cases (original magnification, ×20). (C) BL cell lines are sensitive to a pan-BCL2 inhibitor compared with single-agent inhibitors. Bars represent the mean of 3 replicates from 1 representative experiment of at least 3 separate experiments, and error bars represent 1 standard deviation.