![Figure 5. The degree of antibody opsonization correlates with the degree of antibody-mediated internalization of PLTs by macrophages. PLTs from healthy individuals were incubated with 2 antibodies (anti-HPA1a [A,C] and anti-pan HLA class I [B,D]) in increasing concentrations (0.1 μg/mL, 1 μg/mL, and 10 μg/mL). As negative controls, PLTs were left unopsonized. Representative plots of 3 individual experiments are depicted (A-B). PLTs were incubated with macrophages, and PLT internalization was quantified. (C-D). A Pearson correlation test was used to determine the association between PLT antibody opsonisation and PLT internalization by macrophages.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/131/1/10.1182_blood-2017-07-799270/4/blood799270f5.jpeg?Expires=1762706283&Signature=WdMsodYF0q8tz~5KCBvGxS0d9I6TaofJcnIlCaivA7HJGWa1UlHHmy5OtdwboLDHyS51k-yI3Zskrb30a3NMSwGLNEKOFlWv5CALsGip8fO-BbIGwxKcMyvIm8XdmxG43JmRdEsm9OZ5EsYbp4H9SFuWh3L8~rf1Qqcupg7JAGPhLyYE2Rv86-xgYAyLXQjOKSotkrzJusSaVhOlkj9shJekjrQhHXciB3vxoSpGpaDdsGDOCtJbQsLLcyemLL-bI2EFoZJ0tHwm~4kvXbHNwcwni9reZk1LzxxtT8hJoB9EbEtRj8BcNaZa0o~0uA9z-PeCA6olSPmQzICQ7mgNAQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Figure 5.
The degree of antibody opsonization correlates with the degree of antibody-mediated internalization of PLTs by macrophages. PLTs from healthy individuals were incubated with 2 antibodies (anti-HPA1a [A,C] and anti-pan HLA class I [B,D]) in increasing concentrations (0.1 μg/mL, 1 μg/mL, and 10 μg/mL). As negative controls, PLTs were left unopsonized. Representative plots of 3 individual experiments are depicted (A-B). PLTs were incubated with macrophages, and PLT internalization was quantified. (C-D). A Pearson correlation test was used to determine the association between PLT antibody opsonisation and PLT internalization by macrophages.
