Figure 2. Host CD151 affects lung localization. (A) Representative photos of fluorescent B16F10 cells (labeled with BCECF-AM) in lungs 6 hours after tail vein injection. (B) Quantitation of fluorescent B16F10 cells in lung left lobes (n = 5). (C) In a separate experiment, fluorescent B16F10 cells were again injected and after 6 hours were counted in whole-lung left lobes (left bars; n = 5) or after 24 hours in cryosections at higher magnification (right bars; n = 5). (D) B16F10 cells labeled with CFSE were injected into mouse tail veins. After 24-72 hours, mice were killed, perfused, lungs were digested, and the percentage of CFSE-labeled cells (relative to total suspended lung cells) was determined using flow cytometry. Error bars indicate means ± SEM. *P < .05. WT indicates wild-type; KO, knockout.
Figure 2

Host CD151 affects lung localization. (A) Representative photos of fluorescent B16F10 cells (labeled with BCECF-AM) in lungs 6 hours after tail vein injection. (B) Quantitation of fluorescent B16F10 cells in lung left lobes (n = 5). (C) In a separate experiment, fluorescent B16F10 cells were again injected and after 6 hours were counted in whole-lung left lobes (left bars; n = 5) or after 24 hours in cryosections at higher magnification (right bars; n = 5). (D) B16F10 cells labeled with CFSE were injected into mouse tail veins. After 24-72 hours, mice were killed, perfused, lungs were digested, and the percentage of CFSE-labeled cells (relative to total suspended lung cells) was determined using flow cytometry. Error bars indicate means ± SEM. *P < .05. WT indicates wild-type; KO, knockout.

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