Figure 3 Lineage specificity of aberrant... | American Society of Hematology

$Figure 3. Lineage specificity of aberrant methylation in JMML patient D360. Hypermethylation of the BMP4 CpG island was analyzed by msDHPLC and bisulfite sequencing in flow-sorted CD14+, CD34+, CD235a+, CD3+, and CD19+ spleen cell fractions. On the left are msDHPLC chromatograms in which dotted lines represent healthy control DNA, black lines methylated control DNA, and red lines sample DNA. In the center are the results of bisulfite sequencing in which horizontal lines represent individual alleles (4 per cell sample); ●, methylated cytosines; and ○, unmethylated cytosines. On the right are the results of genomic sequencing; the arrow indicates the position of the heterozygous KRAS c.35 G>T mutation.$

Figure 3

Lineage specificity of aberrant methylation in JMML patient D360. Hypermethylation of the BMP4 CpG island was analyzed by msDHPLC and bisulfite sequencing in flow-sorted CD14⁺, CD34⁺, CD235a⁺, CD3⁺, and CD19⁺ spleen cell fractions. On the left are msDHPLC chromatograms in which dotted lines represent healthy control DNA, black lines methylated control DNA, and red lines sample DNA. In the center are the results of bisulfite sequencing in which horizontal lines represent individual alleles (4 per cell sample); ●, methylated cytosines; and ○, unmethylated cytosines. On the right are the results of genomic sequencing; the arrow indicates the position of the heterozygous KRAS c.35 G>T mutation.

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