Comparison of tumor-induced cytokine profile in B16-GM vaccinated mice. Quantification of cytokines release by ELISA in cell suspension supernatants from spleen of B16-GM immunized C57BL/6 mice (■) or knockouts (A) GM-CSF^−/− (B) βc^−/− and (C) IL-5^−/− mice (□), cocultured with irradiated B16 cells during 5 days, in the presence of IL-2. Values are in nanograms per milliliter. Error bars represent the standard deviation from triplicate samples of 1 single experiment. Similar results were obtained on 3 independent experiments.