Fig. 7. Frequency of allospecific CTL precursors is affected by the culture time-span. / (A) MLR of PBLs (squares), standard 14-day cultured RV cells (triangles), and 28-day cultured RV cells (circles; RV 28) against fully mismatched PBMCs was performed. After 1 round of in vitro stimulation, effector cells were tested against the same allogeneic target (closed symbols) and autologous PHA-derived lymphocytes (open symbols) in a standard cytotoxicity assay, performed in cold inhibition. The frequency of CD8+ T-cell precursors elicited in PBLs (B), RV cells (C), and RV 28 cells (D) with 1 round of stimulation with fully mismatched PBMCs was measured by stimulating the effectors in limiting dilution numbers and by performing a cytotoxic assay. Regression curves were interpolated, and precursor frequencies were determined according to Poisson statistics. Precursor frequencies are shown.
Fig. 7.

Frequency of allospecific CTL precursors is affected by the culture time-span.

(A) MLR of PBLs (squares), standard 14-day cultured RV cells (triangles), and 28-day cultured RV cells (circles; RV 28) against fully mismatched PBMCs was performed. After 1 round of in vitro stimulation, effector cells were tested against the same allogeneic target (closed symbols) and autologous PHA-derived lymphocytes (open symbols) in a standard cytotoxicity assay, performed in cold inhibition. The frequency of CD8+ T-cell precursors elicited in PBLs (B), RV cells (C), and RV 28 cells (D) with 1 round of stimulation with fully mismatched PBMCs was measured by stimulating the effectors in limiting dilution numbers and by performing a cytotoxic assay. Regression curves were interpolated, and precursor frequencies were determined according to Poisson statistics. Precursor frequencies are shown.

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