Western blot analysis of TF antigen in adipose tissues of the stressed obese mice.

Six-week-old male obese mice and their lean counterparts were stressed in restraint tubes for 2 or 20 hours. Mice were killed and epididymal fat tissues were removed and their lysates prepared. Two micrograms of each lysate was loaded on an 8% polyacrylamide gel, and TF antigen was analyzed by Western blotting with the use of polyclonal rabbit antimouse TF antibody. Lanes 1-3, lean mice (1, no stress; 2, 2 hours of stress; 3, 20 hours of stress); lanes 4-6, obese mice (4, no stress; 5, 2 hours of stress; 6, 20 hours of stress). The numbers to the left of the blots indicate molecular weight.